Article
Visual DNA microarray for detection of epidermal growth factor receptor (EGFR) gene mutations.
Scandinavian journal of clinical and laboratory investigation - 1 Nov 2014
Xue Li, Fei Jing-Jing, Song Yi, Xu Rong-Hua, Bai Yu-Jie
Abstract excerpt
OBJECTIVE: To develop a gold nanoparticle-based visual DNA microarray for simple and rapid screening of EGFR gene mutations. METHODS: The DNA fragments contain epidermal growth factor receptor (EGFR) exons 18, 19, 20 and 21 were amplified by polymerase chain reaction (PCR) using biotin-modified primers. The amino-modified oligonucleotides were immobilized on glass surface, which were used as the capturing probes...
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