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Visual gene diagnosis of HBV and HCV based on nanoparticle probe amplification and silver staining enhancement

9 Apr 2003

Abstract excerpt

A visual gene-detecting technique using nanoparticle-supported gene probes is described. With the aid of gold nanoparticle-supported 3'-end-mercapto-derivatized oligonucleotide serving as detection probe, and 5'-end -amino-derivatized oligonucleotide immobilized on glass surface acting as capturing probe, target DNA was detected visually by sandwich hybridization based on highly sensitive "nano-amplification" and...

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PMID: 12696106
DOI: 10.1002/jmv.10379

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