Differential regulation by interleukin-4 and interferon-gamma of an autologous melanoma-specific cytotoxic T-cell clone and the tumor-infiltrating lymphocytes from which it was established.

Abstract excerpt

To investigate the specificity of human tumor-infiltrating lymphocytes (TIL) against autologous tumors, TIL from five metastatic melanoma patients were expanded with rIL-2 and assessed for cytotoxicity in chromium release assays. TIL from a patient showing preferential cytotoxicity against autologous melanoma cells were further analysed. TIL were cloned by limiting dilution. Four out of 27 clones showed...

Topics

• Antibodies, Monoclonal
• Cytotoxicity, Immunologic
• Humans
• Interferon-gamma
• Interleukin-4
• Killer Cells, Lymphokine-Activated
• Lymphocyte Activation
• Lymphocytes
• Melanoma
• Phenotype