Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Fluorophore targeting to cellular proteins via enzyme-mediated azide ligation and strain-promoted cycloaddition.

Journal of the American Chemical Society - 29 Feb 2012

Yao Jennifer Z, Uttamapinant Chayasith, Poloukhtine Andrei, Baskin Jeremy M, Codelli Julian A, Sletten Ellen M, Bertozzi Carolyn R, Popik Vladimir V, Ting Alice Y

Abstract excerpt

Methods for targeting of small molecules to cellular proteins can allow imaging with fluorophores that are smaller, brighter, and more photostable than fluorescent proteins. Previously, we reported targeting of the blue fluorophore coumarin to cellular proteins fused to a 13-amino acid recognition sequence (LAP), catalyzed by a mutant of the Escherichia coli enzyme lipoic acid ligase (LplA). Here, we extend...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Azides
Share to Biocatalysis

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Chlorocebus aethiops

Share to Cyclization

Share to Fluorescent Dyes

Share to Ligases

Share to Luminescent Proteins

Share to Molecular Structure

Source

Open in PubMed Open full text

Identifiers

PMID: 22239252
DOI: 10.1021/ja208090p
PMCID: PMC3306817

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Fluorophore targeting to cellular proteins via enzyme-mediated azide ligation and strain-promoted cycloaddition.PMID 22239252Read ↗

Two-step protein labeling by using lipoic acid ligase with norbornene substrates and subsequent inverse-electron demand Diels-Alder reaction.Semantic proximity Read ↗

Imaging protein-protein interactions inside living cells via interaction-dependent fluorophore ligation.Semantic proximity Read ↗

An engineered aryl azide ligase for site-specific mapping of protein-protein interactions through photo-cross-linking.Semantic proximity Read ↗

Multicolor protein labeling in living cells using mutant β-lactamase-tag technology.Semantic proximity Read ↗

Two-step protein labeling utilizing lipoic acid ligase and Sonogashira cross-coupling.Semantic proximity Read ↗

ChemCell: Chemical Tethering of Large Biomolecules to Cell Surfaces through Diels-Alder LigationSemantic proximity Read ↗

Rapid, randomized development of genetically encoded FRET sensors for small molecules.Semantic proximity Read ↗

Library-on-Library Intercellular Labeling for Selection of Biotin Ligase and Acceptor Peptides.Semantic proximity Read ↗

Engineered peptide ligases for cell signaling and bioconjugation.Semantic proximity Read ↗

Improved Chemical-Genetic Fluorescent Markers for Live Cell Microscopy.Semantic proximity Read ↗

Click Lipid Nanoparticles for the Delivery of mRNA to Metabolically Labeled Cancer CellsSemantic proximity Read ↗

A Bioorthogonal Click Chemistry Toolbox for Targeted Synthesis of Branched and Well-Defined Protein-Protein Conjugates.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.