Article
Phage ORF family recombinases: conservation of activities and involvement of the central channel in DNA binding.
PloS one - 1 Jan 2014
Curtis Fiona A, Malay Ali D, Trotter Alexander J, Wilson Lindsay A, Barradell-Black Michael M H, Bowers Laura Y, Reed Patricia, Hillyar Christopher R T, Yeo Robert P, Sanderson John M, Heddle Jonathan G, Sharples Gary J
Abstract excerpt
Genetic and biochemical evidence suggests that λ Orf is a recombination mediator, promoting nucleation of either bacterial RecA or phage Redβ recombinases onto single-stranded DNA (ssDNA) bound by SSB protein. We have identified a diverse family of Orf proteins that includes representatives implicated in DNA base flipping and those fused to an HNH endonuclease domain. To confirm a functional relationship with the...
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