Article
The C-terminus of the phage λ Orf recombinase is involved in DNA binding.
Journal of molecular recognition : JMR - 1 Jan 2000
Curtis Fiona A, Reed Patricia, Wilson Lindsay A, Bowers Laura Y, Yeo Robert P, Sanderson John M, Walmsley Adrian R, Sharples Gary J
Abstract excerpt
Phage λ Orf substitutes for the activities of the Escherichia coli RecFOR proteins in vivo and is therefore implicated as a recombination mediator, encouraging the assembly of bacterial RecA onto single-stranded DNA (ssDNA) coated with SSB. Orf exists as a dimer in solution, associates with E. coli SSB and binds preferentially to ssDNA. To help identify interacting domains we analysed Orf and SSB proteins...
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