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Article

Method for identifying phosphorylated substrates of specific cyclin/cyclin-dependent kinase complexes.

Proceedings of the National Academy of Sciences of the United States of America - 5 Aug 2014

Li Yinyin, Cross Frederick R, Chait Brian T

Abstract excerpt

In eukaryotes, cell cycle progression is controlled by cyclin/cyclin-dependent kinase (CDK) pairs. To better understand the details of this process, it is necessary to dissect the CDK's substrate pool in a cyclin- and cell cycle stage-specific way. Here, we report a mass spectrometry-based method that couples rapid isolation of native kinase-substrate complexes to on-bead phosphorylation with heavy-labeled ATP...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 25049391
DOI: 10.1073/pnas.1409666111
PMCID: PMC4128132

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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