Article
A cell cycle phosphoproteome of the yeast centrosome.
Science (New York, N.Y.) - 24 Jun 2011
Keck Jamie M, Jones Michele H, Wong Catherine C L, Binkley Jonathan, Chen Daici, Jaspersen Sue L, Holinger Eric P, Xu Tao, Niepel Mario, Rout Michael P, Vogel Jackie, Sidow Arend, Yates John R, Winey Mark
Abstract excerpt
Centrosomes organize the bipolar mitotic spindle, and centrosomal defects cause chromosome instability. Protein phosphorylation modulates centrosome function, and we provide a comprehensive map of phosphorylation on intact yeast centrosomes (18 proteins). Mass spectrometry was used to identify 297 phosphorylation sites on centrosomes from different cell cycle stages. We observed different modes of...
Topics
- Binding Sites
- CDC2 Protein Kinase
- Cell Cycle
- Centrosome
- Cytoskeletal Proteins
- Fungal Proteins
- Fungi
- G1 Phase
- Mitosis
- Mutation
- Phosphoproteins