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Article

Concordance between allele-specific PCR and ultra-deep pyrosequencing for the detection of HIV-1 non-nucleoside reverse transcriptase inhibitor resistance mutations.

Journal of virological methods - 1 Oct 2014

Hunt Gillian M, Morris Lynn, Moorthy Anitha, Coovadia Ashraf, Abrams Elaine J, Strehlau Renate, Kuhn Louise, Persaud Deborah

Abstract excerpt

Recent advances in genotyping technologies have allowed for detection of HIV-1 drug resistance mutations present at low levels. The presence and percentage of Y181C and K103N drug-resistant variants in the blood of 105 subtype C HIV-infected infants who failed single-dose nevirapine prophylaxis for HIV transmission were compared using two highly sensitive genotyping methods, allele-specific PCR (AS-PCR) and...

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Share to Drug Resistance, Viral
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Share to Infant, Newborn

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Share to Mutation, Missense

Source

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Identifiers

PMID: 25034127
DOI: 10.1016/j.jviromet.2014.07.010
PMCID: PMC4150030

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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