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Article

Expansion of human mesenchymal stromal cells from fresh bone marrow in a 3D scaffold-based system under direct perfusion.

PloS one - 1 Jan 2014

Papadimitropoulos Adam, Piccinini Elia, Brachat Sophie, Braccini Alessandra, Wendt David, Barbero Andrea, Jacobi Carsten, Martin Ivan

Abstract excerpt

Mesenchymal stromal/stem cell (MSC) expansion in conventional monolayer culture on plastic dishes (2D) leads to progressive loss of functionality and thus challenges fundamental studies on the physiology of skeletal progenitors, as well as translational applications for cellular therapy and molecular medicine. Here we demonstrate that 2D MSC expansion can be entirely bypassed by culturing freshly isolated bone...

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Identifiers

PMID: 25020062
DOI: 10.1371/journal.pone.0102359
PMCID: PMC4096512

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MSC

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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