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Culture, Expansion and Differentiation of Human Bone Marrow Stromal Cells.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021

Bisio Valeria, Espéli Marion, Balabanian Karl, Anginot Adrienne

Abstract excerpt

Mesenchymal stromal cells (MSC) are a rare, heterogeneous and multipotent population that can be isolated from several tissues. MSC were originally discovered in the bone marrow and studied for their capacity to maintain hematopoietic cells. We will describe here methods to isolate, culture, and bank MSC from human bone marrow. Then, characterization protocols by flow cytometry, clonogenic assays and doubling...

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PMID: 34057710
DOI: 10.1007/978-1-0716-1425-9_1

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