Article
A genetic approach for analyzing the co-operative function of the tRNA mimicry complex, eRF1/eRF3, in translation termination on the ribosome.
Nucleic acids research - 1 Jul 2014
Wada Miki, Ito Koichi
Abstract excerpt
During termination of translation in eukaryotes, a GTP-binding protein, eRF3, functions within a complex with the tRNA-mimicking protein, eRF1, to decode stop codons. It remains unclear how the tRNA-mimicking protein co-operates with the GTPase and with the functional sites on the ribosome. In order to elucidate the molecular characteristics of tRNA-mimicking proteins involved in stop codon decoding, we have...
Topics
- Codon, Terminator
- Fungal Proteins
- Genetic Complementation Test
- Models, Molecular
- Molecular Mimicry
- Mutation
- Peptide Chain Termination, Translational
- Peptide Termination Factors
- Pneumocystis carinii
- Ribosomes
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins