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DNA bar coding and pyrosequencing to analyze adverse events in therapeutic gene transfer

14 Apr 2008

Abstract excerpt

Gene transfer has been used to correct inherited immunodeficiencies, but in several patients integration of therapeutic retroviral vectors activated proto-oncogenes and caused leukemia. Here, we describe improved methods for characterizing integration site populations from gene transfer studies using DNA bar coding and pyrosequencing. We characterized 160,232 integration site sequences in 28 tissue samples from...

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PMID: 18411205
DOI: 10.1093/nar/gkn125

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