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Mutations in the palm subdomain of Twa DNA polymerase to enhance PCR efficiency and its function analysis.

Journal of biotechnology - 20 Aug 2014

Cho Sung Suk, Yu Mi, Kwon Suk-Tae

Abstract excerpt

Among the family B DNA polymerases, the Twa DNA polymerase from T. wiotapuensis, a hyperthermophilic archaeon, has exceedingly high fidelity. For applications in PCR, however, the enzyme is limited by its low extension rate and processivity. To resolve these weaknesses, we focused on two amino acid residues (A381 and N501) located at the palm subdomain of Twa DNA polymerase. Following replacement of these...

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PMID: 24865518
DOI: 10.1016/j.jbiotec.2014.05.007

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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