Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Structural mechanism for coordination of proofreading and polymerase activities in archaeal DNA polymerases.

Journal of molecular biology - 12 Aug 2005

Kuroita Toshihiro, Matsumura Hiroyoshi, Yokota Naohiko, Kitabayashi Masao, Hashimoto Hiroshi, Inoue Tsuyoshi, Imanaka Tadayuki, Kai Yasushi

Abstract excerpt

A novel mechanism for controlling the proofreading and polymerase activities of archaeal DNA polymerases was studied. The 3'-5'exonuclease (proofreading) activity and PCR performance of the family B DNA polymerase from Thermococcus kodakaraensis KOD1 (previously Pyrococcus kodakaraensis KOD1) were altered efficiently by mutation of a "unique loop" in the exonuclease domain. Interestingly, eight different H147...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Archaeal Proteins
Share to Cattle

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Crystallography, X-Ray

Share to DNA-Directed DNA Polymerase

Share to Mutagenesis, Site-Directed

Share to Protein Conformation

Share to Protein Structure, Tertiary

Share to Static Electricity

Source

Identifiers

PMID: 16019029
DOI: 10.1016/j.jmb.2005.06.015

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Structural mechanism for coordination of proofreading and polymerase activities in archaeal DNA polymerases.PMID 16019029Read ↗

A DNA binding motif coordinating synthesis and degradation in proofreading DNA polymerases.Semantic proximity Read ↗

Aromatic residues located close to the active center are essential for the catalytic reaction of flap endonuclease-1 from hyperthermophilic archaeon Pyrococcus horikoshii.Semantic proximity Read ↗

Crystal structure of the Pyrococcus horikoshii DNA primase-UTP complex: implications for the mechanism of primer synthesis.Semantic proximity Read ↗

Distal Mutations in the β-Clamp of DNA Polymerase III* Disrupt DNA Orientation and Affect Exonuclease Activity.Semantic proximity Read ↗

Novel ribonucleotide discrimination in the RNA polymerase-like two-barrel catalytic core of Family D DNA polymerases.Semantic proximity Read ↗

Structural basis for substrate recognition and processive cleavage mechanisms of the trimeric exonuclease PhoExo I.Semantic proximity Read ↗

Mutations to create thermostable reverse transcriptase with bacterial family A DNA polymerase from Thermotoga petrophila K4.Semantic proximity Read ↗

Structural Studies of HNA Substrate Specificity in Mutants of an Archaeal DNA Polymerase Obtained by Directed Evolution.Semantic proximity Read ↗

Structure of an open conformation of T7 DNA polymerase reveals novel structural features regulating primer-template stabilization at the polymerization active site.Semantic proximity Read ↗

ø29 DNA polymerase residue Lys383, invariant at motif B of DNA-dependent polymerases, is involved in dNTP binding.Semantic proximity Read ↗

Structure of HsdS subunit from Thermoanaerobacter tengcongensis sheds lights on mechanism of dynamic opening and closing of type I methyltransferase.Semantic proximity Read ↗

A conserved polar residue plays a critical role in mismatch detection in A-family DNA polymerases.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.