Article
Evolutionary conservation of residues in vertebrate DNA polymerase N conferring low fidelity and bypass activity.
Nucleic acids research - 1 Jun 2010
Takata Kei-ichi, Arana Mercedes E, Seki Mineaki, Kunkel Thomas A, Wood Richard D
Abstract excerpt
POLN is a nuclear A-family DNA polymerase encoded in vertebrate genomes. POLN has unusual fidelity and DNA lesion bypass properties, including strong strand displacement activity, low fidelity favoring incorporation of T for template G and accurate translesion synthesis past a 5S-thymine glycol (5S-Tg). We searched for conserved features of the polymerase domain that distinguish it from prokaryotic pol I-type DNA...
Topics
- Amino Acid Motifs
- DNA-Directed DNA Polymerase
- Evolution, Molecular
- Humans
- Lysine
- Mutation
- Protein Structure, Tertiary
- Tyrosine