Article
The amino terminal extension of mammalian mitochondrial RNA polymerase ensures promoter specific transcription initiation.
Nucleic acids research - 1 Apr 2014
Posse Viktor, Hoberg Emily, Dierckx Anke, Shahzad Saba, Koolmeister Camilla, Larsson Nils-Göran, Wilhelmsson L Marcus, Hällberg B Martin, Gustafsson Claes M
Abstract excerpt
Mammalian mitochondrial transcription is executed by a single subunit mitochondrial RNA polymerase (Polrmt) and its two accessory factors, mitochondrial transcription factors A and B2 (Tfam and Tfb2m). Polrmt is structurally related to single-subunit phage RNA polymerases, but it also contains a unique N-terminal extension (NTE) of unknown function. We here demonstrate that the NTE functions together with Tfam to...
Topics
- Animals
- DNA-Binding Proteins
- DNA-Directed RNA Polymerases
- High Mobility Group Proteins
- Humans
- Methyltransferases
- Mice
- Mitochondria
- Mitochondrial Proteins
- Mutation
- Promoter Regions, Genetic
- Protein Structure, Tertiary