Article
Transcription inhibition by the depsipeptide antibiotic salinamide A.
eLife - 30 Apr 2014
Degen David, Feng Yu, Zhang Yu, Ebright Katherine Y, Ebright Yon W, Gigliotti Matthew, Vahedian-Movahed Hanif, Mandal Sukhendu, Talaue Meliza, Connell Nancy, Arnold Eddy, Fenical William, Ebright Richard H
Abstract excerpt
We report that bacterial RNA polymerase (RNAP) is the functional cellular target of the depsipeptide antibiotic salinamide A (Sal), and we report that Sal inhibits RNAP through a novel binding site and mechanism. We show that Sal inhibits RNA synthesis in cells and that mutations that confer Sal-resistance map to RNAP genes. We show that Sal interacts with the RNAP active-center 'bridge-helix cap' comprising the...
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