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Article

Comparative next-generation sequencing of adeno-associated virus inverted terminal repeats.

BioTechniques - 1 May 2014

Petri Karl, Fronza Raffaele, Gabriel Richard, Käppel Christine, Nowrouzi Ali, Linden R Michael, Henckaerts Els, Schmidt Manfred

Abstract excerpt

The inverted terminal repeats (ITRs) of adeno-associated virus (AAV) are notoriously difficult to sequence owing to their high GC-content (70%) and palindromic sequences that result in the formation of a very stable, 125 bp long, T-shaped hairpin structure. Here we evaluate the performance of two widely used next-generation sequencing platforms, 454 GS FLX (Roche) and MiSeq Benchtop Sequencer (Illumina), in...

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PMID: 24806228
DOI: 10.2144/000114170

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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