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Article

Increased single-nucleotide discrimination of PCR by primer probes bearing hydrophobic 4'C modifications.

Chembiochem : a European journal of chemical biology - 5 Mar 2004

Strerath Michael, Gaster Jens, Summerer Daniel, Marx Andreas

Abstract excerpt

We report on significantly increased selectivity of real-time PCR through employment of primer probes that bear hydrophobic 4'C modifications at the 3'-terminal nucleotide. The primer probes were designed to bind the target sequences in such a way that the 3'-terminal nucleotide defines whether a matched or a single mismatched basepair is present depending on the respective target sequence. Several commercially...

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PMID: 14997525
DOI: 10.1002/cbic.200300757

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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