Article
Harnessing asymmetrical substrate recognition by thermostable EndoV to achieve balanced linear amplification in multiplexed SNP typing.
Biochemistry and cell biology = Biochimie et biologie cellulaire - 1 Apr 2006
Turner Daniel J, Pingle Maneesh R, Barany Francis
Abstract excerpt
Multiplexed amplification of specific DNA sequences, by PCR or by strand-displacement amplification, is an intrinsically biased process. The relative abundance of amplified DNA can be altered significantly from the original representation and, in extreme cases, allele dropout can occur. In this paper, we present a method of linear amplification of DNA that relies on the cooperative, sequence-dependent functioning...
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