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Enrichment of cells with TALEN-induced mutations using surrogate reporters.

Methods (San Diego, Calif.) - 15 Aug 2014

Kim Young-Hoon, Ramakrishna Suresh, Kim Hyongbum, Kim Jin-Soo

Abstract excerpt

Targeted gene knockout using engineered nucleases such as transcription activator like-effector nucleases (TALENs) is a gold standard for investigating the functions of a gene of interest. Although most TALENs can cleave chromosomal DNA efficiently, the activities of designed TALENs are not always high enough to allow the efficient derivation of cells containing TALEN-driven mutations. Thus, simple methods to...

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Identifiers

PMID: 24780521
DOI: 10.1016/j.ymeth.2014.04.012

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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