Magnetic separation and antibiotics selection enable enrichment of cells with ZFN/TALEN-induced mutations.

Abstract excerpt

The ability to enrich cells with targeted mutations greatly facilitates the process of using engineered nucleases, including zinc-finger nucleases and transcription activator-like effector nucleases, to construct such cells. We previously used surrogate reporters to enrich cells containing nuclease-induced mutations via flow cytometry. This method is, however, limited by the availability of flow cytometers....

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