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Article

Surrogate reporters for enrichment of cells with nuclease-induced mutations.

Nature methods - 9 Oct 2011

Kim Hyojin, Um Eunji, Cho Sung-Rae, Jung Chorong, Kim Hyongbum, Kim Jin-Soo

Abstract excerpt

Zinc-finger nucleases (ZFNs) and TAL-effector nucleases (TALENs) are powerful tools for creating genetic modifications in eukaryotic cells and organisms. But wild-type and mutant cells that contain genetic modifications induced by these programmable nucleases are often phenotypically indistinguishable, hampering isolation of mutant cells. Here we show that transiently transfected episomal reporters encoding...

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PMID: 21983922
DOI: 10.1038/nmeth.1733

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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