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Article

[Construction of pcDNA3-HERG-G572R expression vector and establishment of a cell line stably expressing HKE-HERG-G572R].

Nan fang yi ke da xue xue bao = Journal of Southern Medical University - 1 Mar 2014

Yang Yang, Huang Na, Gao Ling, Chang Su'e, Guo Bo, Hu Lili, Song Tusheng, Huang Chen

Abstract excerpt

OBJECTIVE: To construct the pcDNA3-HERG-G572R expression vector and establish a cell line stably expressing HKE-HERG-G572R. METHODS: HERG-G572R mutant fragment was constructed by over-lap extension PCR and validated by DNA sequencing. The HKE-HERG-G572R expression vector was constructed and transfected into HEK293 cells to obtain a cell line stably expressing HKE-HERG-G572R. RESULTS: The pcDNA3-HERG-G572R...

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PMID: 24670439

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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