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Article

[Construction of different mutants of HA-tagged human RAGE gene and their eukaryotic expression].

Nan fang yi ke da xue xue bao = Journal of Southern Medical University - 1 Oct 2008

Cheng Wei-wei, Li Yu-sheng, Gong Xiao-wei, Zhao Lin-lin, Wang Ji-gang, Deng Peng, Jiang Yong

Abstract excerpt

OBJECTIVE: To construct eukaryotic expression vectors for HA-tagged receptor for advanced glycation end products (RAGE) mutants. METHODS: Site-directed mutagenesis was applied to wild-type RAGE gene cloned in the pcDNA3 vector with HA tag to obtain the mutants pcDNA3-HA-RAGE(S391A), pcDNA3-HA-RAGE(S399A), pcDNA3-HA-RAGE(S400A), and pcDNA3-HA-RAGE(T401A). After identification by sequencing, the mutants were...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 18971170

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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