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Article

Quantitative live-cell imaging reveals spatio-temporal dynamics and cytoplasmic assembly of the 26S proteasome.

Nature communications - 6 Mar 2014

Pack Chan-Gi, Yukii Haruka, Toh-e Akio, Kudo Tai, Tsuchiya Hikaru, Kaiho Ai, Sakata Eri, Murata Shigeo, Yokosawa Hideyoshi, Sako Yasushi, Baumeister Wolfgang, Tanaka Keiji, Saeki Yasushi

Abstract excerpt

The 26S proteasome is a 2.5-MDa multisubunit protease complex that degrades polyubiquitylated proteins. Although its functions and structure have been extensively characterized, little is known about its dynamics in living cells. Here, we investigate the absolute concentration, spatio-temporal dynamics and complex formation of the proteasome in living cells using fluorescence correlation spectroscopy. We find...

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Identifiers

PMID: 24598877
DOI: 10.1038/ncomms4396

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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