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Article

High-resolution mapping of epitopes on the C2 domain of factor VIII by analysis of point mutants using surface plasmon resonance.

Blood - 24 Apr 2014

Nguyen Phuong-Cac T, Lewis Kenneth B, Ettinger Ruth A, Schuman Jason T, Lin Jasper C, Healey John F, Meeks Shannon L, Lollar Pete, Pratt Kathleen P

Abstract excerpt

Neutralizing anti-factor VIII (FVIII) antibodies that develop in patients with hemophilia A and in murine hemophilia A models, clinically termed "inhibitors," bind to several distinct surfaces on the FVIII-C2 domain. To map these epitopes at high resolution, 60 recombinant FVIII-C2 proteins were generated, each having a single surface-exposed residue mutated to alanine or a conservative substitution. The binding...

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Identifiers

PMID: 24591205
DOI: 10.1182/blood-2013-09-527275
PMCID: PMC3999758

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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