Article
Acyl-chain remodeling of dioctanoyl-phosphatidylcholine in Saccharomyces cerevisiae mutant defective in de novo and salvage phosphatidylcholine synthesis.
Biochemical and biophysical research communications - 7 Mar 2014
Kishino Hideyuki, Eguchi Hiroki, Takagi Keiko, Horiuchi Hiroyuki, Fukuda Ryouichi, Ohta Akinori
Abstract excerpt
A yeast strain, in which endogenous phosphatidylcholine (PC) synthesis is controllable, was constructed by the replacement of the promoter of PCT1, encoding CTP:phosphocholine cytidylyltransferase, with GAL1 promoter in a double deletion mutant of PEM1 and PEM2, encoding phosphatidylethanolamine methyltransferase and phospholipid methyltransferase, respectively. This mutant did not grow in the glucose-containing...
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