Article
A computational method for estimating the PCR duplication rate in DNA and RNA-seq experiments.
BMC bioinformatics - 14 Mar 2017
Bansal Vikas
Abstract excerpt
BACKGROUND: PCR amplification is an important step in the preparation of DNA sequencing libraries prior to high-throughput sequencing. PCR amplification introduces redundant reads in the sequence data and estimating the PCR duplication rate is important to assess the frequency of such reads. Existing computational methods do not distinguish PCR duplicates from "natural" read duplicates that represent independent...
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