Article
Recursive genomewide recombination and sequencing reveals a key refinement step in the evolution of a metabolic innovation in Escherichia coli.
Proceedings of the National Academy of Sciences of the United States of America - 11 Feb 2014
Quandt Erik M, Deatherage Daniel E, Ellington Andrew D, Georgiou George, Barrick Jeffrey E
Abstract excerpt
Evolutionary innovations often arise from complex genetic and ecological interactions, which can make it challenging to understand retrospectively how a novel trait arose. In a long-term experiment, Escherichia coli gained the ability to use abundant citrate (Cit(+)) in the growth medium after ∼31,500 generations of evolution. Exploiting this previously untapped resource was highly beneficial: later Cit(+)...
Topics
- Carrier Proteins
- Escherichia coli
- Evolution, Molecular
- Genome, Bacterial
- Mutation
- Recombination, Genetic