Article
Generation of a useful roX1 allele by targeted gene conversion.
G3 (Bethesda, Md.) - 10 Jan 2014
Apte Manasi S, Moran Victoria A, Menon Debashish U, Rattner Barbara P, Barry Kathryn Hughes, Zunder Rachel M, Kelley Richard, Meller Victoria H
Abstract excerpt
Methods for altering the sequence of endogenous Drosophila melanogaster genes remain labor-intensive. We have tested a relatively simple strategy that enables the introduction of engineered mutations in the vicinity of existing P-elements. This method was used to generate useful alleles of the roX1 gene, which produces a noncoding RNA involved in dosage compensation. The desired change was first introduced into a...
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