Article
Improvement of the efficiency of transglycosylation catalyzed by α-galactosidase from Thermotoga maritima by protein engineering.
Biochemistry. Biokhimiia - 1 Oct 2013
Bobrov K S, Borisova A S, Eneyskaya E V, Ivanen D R, Shabalin K A, Kulminskaya A A, Rychkov G N
Abstract excerpt
At high concentrations of p-nitrophenyl-α-D-galactopyranoside (pNPGal) as a substrate, its hydrolysis catalyzed by α-galactosidase from Thermotoga maritima (TmGalA) is accompanied by transglycosylation resulting in production of a mixture of (α1,2)-, (α1,3)-, and (α1,6)-p-nitrophenyl (pNP)-digalactosides. Molecular modeling of the reaction stage preceding the formation of the pNP-digalactosides within the active...
Topics
- Biocatalysis
- Glycosylation
- Hydrolysis
- Models, Molecular
- Mutation
- Nitrophenylgalactosides
- Protein Engineering
- Thermotoga maritima
- alpha-Galactosidase