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Article

Cloning and characterization of Thermotoga maritima beta-glucuronidase.

Carbohydrate research - 16 Jan 2006

Salleh Hamzah M, Müllegger Johannes, Reid Stephen P, Chan Wing Y, Hwang Jiyoung, Warren R Antony J, Withers Stephen G

Abstract excerpt

The putative beta-glucuronidase from Thermotoga maritima, comprising 563 amino acid residues conjugated with a Hisx6 tag, was cloned and expressed in Escherichia coli. The enzyme has a moderately broad specificity, hydrolysing a range of p-nitrophenyl glycoside substrates, but has greatest activity on p-nitrophenyl beta-D-glucosiduronic acid (kcat=68 s(-1), kcat/K(M)= 4.5x10(5) M(-1) s(-1)). The enzyme also shows...

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PMID: 16303119
DOI: 10.1016/j.carres.2005.10.005

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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