Article
Improved oligosaccharide synthesis by protein engineering of beta-glucosidase CelB from hyperthermophilic Pyrococcus furiosus.
Biotechnology and bioengineering - 5 May 2001
Hansson T, Kaper T, van Der Oost J, de Vos W M, Adlercreutz P
Abstract excerpt
Enzymatic transglycosylation of lactose into oligosaccharides was studied using wild-type beta-glucosidase (CelB) and active site mutants thereof (M424K, F426Y, M424K/F426Y) and wild-type beta-mannosidase (BmnA) of the hyperthermophilic Pyrococcus furiosus. The effects of the mutations on kinetics, enzyme activity, and substrate specificity were determined. The oligosaccharide synthesis was carried out in aqueous...
Topics
- Amino Acid Sequence
- Hydrolases
- Molecular Sequence Data
- Mutation
- Oligosaccharides
- Phosphoenolpyruvate Sugar Phosphotransferase System
- Protein Engineering
- Pyrococcus furiosus
- Sequence Homology, Amino Acid
- Substrate Specificity
- beta-Glucosidase