Article
Detection of K-ras oncogene from the human genomic DNA using ultrasonication and a quantum dots-based microfluidic chip.
Journal of nanoscience and nanotechnology - 1 Sept 2013
Noh Han Na, Kim Jong Sung
Abstract excerpt
In clinical diagnostics, single-stranded DNAs (ssDNA) have been prepared from the human genomic DNA for the detection of a specific gene. In this study, the human genomic DNA was degraded via ultrasonication in solution, and K-ras oncogene was detected from the DNA fragments via the fluorescence quenching of quantum dots (QDs) by intercalating dyes after hybridization of the target, to probe DNAs in a...
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