A microfluidic chip-based fluorescent biosensor for the sensitive and specific detection of label-free single-base mismatch via magnetic beads-based "sandwich" hybridization strategy.

Abstract excerpt

A novel microfluidic chip-based fluorescent DNA biosensor, which utilized the electrophoretic driving mode and magnetic beads-based "sandwich" hybridization strategy, was developed for the sensitive and ultra-specific detection of single-base mismatch DNA in this study. In comparison with previous biosensors, the proposed DNA biosensor has much more robust resistibility to the complex matrix of real saliva and...

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