Article
High-Throughput DNA Array for SNP Detection of KRAS Gene Using a Centrifugal Microfluidic Device.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2016
Sedighi Abootaleb, Li Paul C H
Abstract excerpt
Here, we describe detection of single nucleotide polymorphism (SNP) in genomic DNA samples using a NanoBioArray (NBA) chip. Fast DNA hybridization is achieved in the chip when target DNAs are introduced to the surface-arrayed probes using centrifugal force. Gold nanoparticles (AuNPs) are used to assist SNP detection at room temperature. The parallel setting of sample introduction in the spiral channels of the NBA...
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