Article
Rapid creation of forward-genetics tools for C. briggsae using TALENs: lessons for nonmodel organisms.
Molecular biology and evolution - 1 Feb 2014
Wei Qing, Shen Yongquan, Chen Xiangmei, Shifman Yelena, Ellis Ronald E
Abstract excerpt
Although evolutionary studies of gene function often rely on RNA interference, the ideal approach would use reverse genetics to create null mutations for cross-species comparisons and forward genetics to identify novel genes in each species. We have used transcription activator-like effector nucleases (TALENs) to facilitate both approaches in Caenorhabditis nematodes. First, by combining golden gate cloning and...
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