Article
Circularly permuted green fluorescent proteins engineered to sense Ca2+.
Proceedings of the National Academy of Sciences of the United States of America - 13 Mar 2001
Nagai T, Sawano A, Park E S, Miyawaki A
Abstract excerpt
To visualize Ca(2+)-dependent protein-protein interactions in living cells by fluorescence readouts, we used a circularly permuted green fluorescent protein (cpGFP), in which the amino and carboxyl portions had been interchanged and reconnected by a short spacer between the original termini. The cpGFP was fused to calmodulin and its target peptide, M13. The chimeric protein, which we have named "pericam," was...
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