Article
In vivo protein stabilization based on fragment complementation and a split GFP system.
Proceedings of the National Academy of Sciences of the United States of America - 16 Nov 2010
Lindman Stina, Hernandez-Garcia Armando, Szczepankiewicz Olga, Frohm Birgitta, Linse Sara
Abstract excerpt
Protein stabilization was achieved through in vivo screening based on the thermodynamic linkage between protein folding and fragment complementation. The split GFP system was found suitable to derive protein variants with enhanced stability due to the correlation between effects of mutations on the stability of the intact chain and the effects of the same mutations on the affinity between fragments of the chain....
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