Rational design and implementation of a chemically inducible heterotrimerization system.

doi:10.1038/s41592-020-0913-x

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Article

Rational design and implementation of a chemically inducible heterotrimerization system.

Nature methods - 1 Sept 2020

Wu Helen D, Kikuchi Masaki, Dagliyan Onur, Aragaki Adam K, Nakamura Hideki, Dokholyan Nikolay V, Umehara Takashi, Inoue Takanari

Abstract excerpt

Chemically inducible dimerization (CID) uses a small molecule to induce binding of two different proteins. CID tools such as the FK506-binding protein-FKBP-rapamycin-binding- (FKBP-FRB)-rapamycin system have been widely used to probe molecular events inside and outside cells. While various CID tools are available, chemically inducible trimerization (CIT) does not exist, due to inherent challenges in designing a...

Topics

HeLa Cells
Humans
Models, Molecular
Mutation
Protein Conformation
Sirolimus
TOR Serine-Threonine Kinases
Tacrolimus Binding Proteins

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PMID: 32747768
DOI: 10.1038/s41592-020-0913-x
PMCID: PMC9936427

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