Article
Alterations to the primer grip of p66 HIV-1 reverse transcriptase and their consequences for template-primer utilization.
Biochemistry - 2 Jul 1996
Ghosh M, Jacques P S, Rodgers D W, Ottman M, Darlix J L, Le Grice S F
Abstract excerpt
Alanine scanning mutagenesis was undertaken to evaluate the structural significance of Met230-His235 of the 66 kDa subunit of p66/p51 human immunodeficiency virus reverse transcriptase (HIV-1 RT). Together with Glu224-Trp229, these residues provide the framework of the p66 "primer grip", whose pr...
Topics
- Amino Acid Sequence
- Base Sequence
- DNA Primers
- DNA-Directed DNA Polymerase
- HIV Reverse Transcriptase
- HIV-1
- Hydrolysis
- Molecular Sequence Data
- Mutation
- RNA-Directed DNA Polymerase
- Recombination, Genetic
- Ribonuclease H
- Sequence Deletion
- Substrate Specificity
- Templates, Genetic