Article
Deletion of carboxy-terminal residues of murine granulocyte-macrophage colony-stimulating factor results in a loss of biologic activity and altered glycosylation.
Archives of biochemistry and biophysics - 1 Jan 1990
LaBranche C C, Clark S C, Johnson G D, Ornstein D, Sabath D E, Tushinski R, Paetkau V, Prystowsky M B
Abstract excerpt
A deletion mutant of murine granulocyte-macrophage colony-stimulating factor (GM-CSF) which differs in primary structure from native GM-CSF in the carboxy-terminal 11 amino acids was prepared. Four amino acid residues are mutated and the seven terminal residues including Cys-118 are deleted. Supe...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Northern
- Cell Differentiation
- Cell Line
- Chromosome Deletion
- Cloning, Molecular
- Colony-Stimulating Factors
- Escherichia coli
- Genes
- Glycoside Hydrolases
- Glycosylation
- Granulocyte-Macrophage Colony-Stimulating Factor