Article
Deletion of carboxy-terminal residues of murine granulocyte-macrophage colony-stimulating factor results in a loss of biologic activity and altered glycosylation.
Archives of biochemistry and biophysics - 1 Jan 1990
LaBranche C C, Clark S C, Johnson G D, Ornstein D, Sabath D E, Tushinski R, Paetkau V, Prystowsky M B
Abstract excerpt
A deletion mutant of murine granulocyte-macrophage colony-stimulating factor (GM-CSF) which differs in primary structure from native GM-CSF in the carboxy-terminal 11 amino acids was prepared. Four amino acid residues are mutated and the seven terminal residues including Cys-118 are deleted. Supernatants from COS-1 cells transfected with this deletion mutant (GM-CSF(del] showed a 3000-fold decrease in the ability...
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