Article
Characterization of the sequence-specific interaction of mouse c-myb protein with DNA.
The EMBO journal - 1 Jan 1990
Howe K M, Reakes C F, Watson R J
Abstract excerpt
We have examined parameters that affect sequence-specific interactions of the mouse c-myb protein with DNA oligomers containing the Myb-binding motif (CA/CGTTPu). Complexes formed between these oligomers and in vitro translated c-myb proteins were analysed by electrophoresis on non-denaturing polyacrylamide gels using the mobility-shift assay. By progressive truncation of c-myb coding sequences it was...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Binding Sites
- DNA
- Electrophoresis, Polyacrylamide Gel
- Mice
- Molecular Sequence Data
- Mutation
- Plasmids
- Polymerase Chain Reaction
- Protein Biosynthesis
