Article
Simultaneous identification of 36 mutations in KRAS codons 61 and 146, BRAF, NRAS, and PIK3CA in a single reaction by multiplex assay kit.
BMC cancer - 3 Sept 2013
Bando Hideaki, Yoshino Takayuki, Shinozaki Eiji, Nishina Tomohiro, Yamazaki Kentaro, Yamaguchi Kensei, Yuki Satoshi, Kajiura Shinya, Fujii Satoshi, Yamanaka Takeharu, Tsuchihara Katsuya, Ohtsu Atsushi
Abstract excerpt
BACKGROUND: Retrospective analyses in the West suggest that mutations in KRAS codons 61 and 146, BRAF, NRAS, and PIK3CA are negative predictive factors for cetuximab treatment in colorectal cancer patients. We developed a novel multiplex kit detecting 36 mutations in KRAS codons 61 and 146, BRAF, NRAS, and PIK3CA using Luminex (xMAP) assay in a single reaction. METHODS: Tumor samples and clinical data from Asian...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.