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Development and extensive analytical validation of deep amplicon sequencing for detecting KRAS and NRAS mutations in metastatic colorectal cancer samples.

Neoplasma - 1 Jan 2022

Slavkovský Rastislav, Stránská Jana, Věnsková Veronika, Jančik Sylwia, Kotková Lucie, Šimová Jarmila, Vojta Petr, Flodr Patrik, Bartoušková Marie, Študentová Hana, Hajdúch Marián, Drábek Jiří

Abstract excerpt

The presence of wild-type RAS alleles, as determined by genotyping codons 12, 13, 59, 61, 117, and 146, is a prerequisite for personalized anti-EGFR treatment of metastatic colorectal cancer (mCRC) patients. Here we describe analytical validation of in-house developed massively parallel sequencing technology (MPS) in comparison to the in vitro diagnostics (IVD) certified qPCR method. DNA extracted from FFPE...

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Identifiers

PMID: 34881628
DOI: 10.4149/neo_2021_210907N1276

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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