Article
Substitution of a single amino acid (aspartic acid for histidine) converts the functional activity of human complement C4B to C4A.
Proceedings of the National Academy of Sciences of the United States of America - 1 Sept 1990
Carroll M C, Fathallah D M, Bergamaschini L, Alicot E M, Isenman D E
Abstract excerpt
The C4B isotype of the fourth component of human complement (C4) displays 3- to 4-fold greater hemolytic activity than does its other isotype C4A. This correlates with differences in their covalent binding efficiencies to erythrocytes coated with antibody and complement C1. C4A binds to a greater...
Topics
- Animals
- Aspartic Acid
- Base Sequence
- Cell Line
- Complement C4a
- Complement C4b
- Genetic Vectors
- Hemolysis
- Histidine
- Humans
- Kinetics
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Plasmids
- Recombinant Proteins
- Transfection