Resolution by diagonal gel mobility shift assays of multisubunit complexes binding to a functionally important element of the rat growth hormone gene promoter.

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Article

Resolution by diagonal gel mobility shift assays of multisubunit complexes binding to a functionally important element of the rat growth hormone gene promoter.

The Journal of biological chemistry - 25 Aug 1990

Schaufele F, Cassill J A, West B L, Reudelhuber T

Abstract excerpt

DNase I footprinting identifies a tissue-general factor, GHF3, binding to the rat growth hormone promoter between nucleotides -239 and -219. Mutation of the GHF3-binding site reduces promoter activity to 30% of that of the wild-type promoter after transfection into GC cells. Southwestern blotting...

Topics

Animals
Base Sequence
Binding Sites
Cell Nucleus
Chromatography, Affinity
Deoxyribonuclease I
Electrophoresis, Gel, Two-Dimensional
Growth Hormone
Immunoblotting
Liver
Macromolecular Substances

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutation

Promoter Regions, Genetic

Rats

Transcription Factors

Transcription, Genetic

Transfection

Source

Open in PubMed

Identifiers

PMID: 2387870

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-2387870-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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