Article
The effects of ethidium bromide and Mg++ ion on strand exchange in the Hin-mediated inversion reaction.
Molecules and cells - 31 Dec 2003
Lee Hee Jung, Lee Young Lang, Ji Jeong Jun, Lim Heon Man
Abstract excerpt
The biochemical reaction of a site-specific recombinase such as Hin invertase or gammadelta resolvase starts with binding of the recombinase to its recombination site and cleavage of the DNA in the center of the site. This is followed by strand exchange and finally ligation of the ends of the recombined strands. Previous biochemical studies have shown that Hin invertase and gammadelta resolvase cannot proceed...
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